One of the newest gene silencing tools is the CRISPR-based technology known as CRISPR interference (CRISPRi). CRISPRi is a modified version of the CRISPR/Cas9 system that allows for targeted gene silencing without making permanent changes to the DNA sequence. CRISPRi works by using a catalytically inactive form of the Cas9 protein, known as “dead” Cas9 […]

Small interfering RNA (siRNA) electroporation is a technique used to introduce siRNA molecules into target cells in order to silence or knockdown the expression of specific genes. The siRNA molecules are designed to complement the target messenger RNA (mRNA), which is then degraded by the RNA-induced silencing complex (RISC), leading to a reduction in protein […]

In vivo refers to biological processes, experiments, or treatments that occur within a living organism, as opposed to in vitro, which refers to studies conducted outside of the organism, usually in a controlled laboratory environment such as a cell culture or test tube. Tissues in vivo are the functional groups of cells and extracellular matrix […]