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DNA Gene Therapy
DNA gene therapy is a therapeutic approach that involves introducing, modifying, or repairing genetic material within a patient’s cells to treat or prevent disease. This method has the potential to address a wide range of genetic disorders, as well as certain viral infections and some types of cancer. DNA gene therapy can involve several different…
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Gene Transfer
Gene transfer refers to the process of introducing foreign genetic material (DNA or RNA) into an organism or a cell. This technique is widely used in biological research and has various applications in gene therapy, agriculture, and biotechnology. Gene transfer can occur naturally or be facilitated by artificial methods. Natural gene transfer mechanisms include: Artificial…
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Reversible Cell Electroporation
Reversible cell electroporation is a technique where an electric field is applied to cells to temporarily increase the permeability of their membranes, allowing the introduction of foreign molecules such as DNA, RNA, or proteins into the cells. The term “reversible” refers to the fact that the changes to the cell membrane are transient, and the…
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Electric Pulse Delivery
Electric pulse delivery, also known as electroporation, is a technique used to introduce foreign molecules, such as DNA, RNA, or proteins, into cells by applying an electric field. This electric field transiently increases the permeability of the cell membrane, creating temporary pores through which the molecules can enter the cell. Electric pulse delivery is widely…
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Cultured Cells
Cultured cells refer to cells that are grown and maintained under controlled conditions outside of their original organism or natural environment. Cell culture is a crucial technique in biological research, allowing scientists to study the behavior, function, and response of cells under different conditions, as well as develop therapies and perform drug testing. There are…
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Gene Transfer Types
Gene transfer refers to the process of introducing foreign genetic material into an organism or a cell. Various gene transfer techniques have been developed, and they can be broadly classified into two categories: horizontal gene transfer and vertical gene transfer. In addition to these natural gene transfer processes, several artificial methods have been developed to…
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DNA Electrophoresis
DNA electrophoresis is a widely used laboratory technique that allows for the separation and analysis of DNA fragments based on their size. The method utilizes an electric field to drive negatively charged DNA molecules through a porous gel matrix, typically made of agarose or polyacrylamide. As the DNA fragments migrate through the gel, smaller fragments…
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Intratumoral Plasmid Electroporation
Intratumoral plasmid electroporation is a method used to deliver plasmid DNA directly into tumor cells within a tumor mass. This technique combines two approaches: intratumoral injection and electroporation. The goal is to facilitate gene transfer into cancer cells to modulate tumor growth, induce apoptosis, or stimulate the immune system to target the tumor. The process…
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Protein Electroporation Buffer
Electroporation is a technique used to introduce foreign DNA or other molecules into cells by applying an electric field. This method increases the permeability of the cell membrane, allowing the molecules to enter the cell more efficiently. Electroporation buffers are solutions that facilitate the electroporation process by providing the appropriate environment for both the cells…
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Anti-inflammatory Cytokines
Anti-inflammatory cytokines are a group of signaling molecules that play a crucial role in regulating inflammation and immune responses in the body. These cytokines help to balance the pro-inflammatory cytokines that promote inflammation and tissue damage. They are essential for maintaining homeostasis and preventing excessive inflammation, which can lead to chronic diseases and tissue damage.…